Designing the focal plane spacing for multifocal plane microscopy
نویسندگان
چکیده
منابع مشابه
Designing the focal plane spacing for multifocal plane microscopy.
Multifocal plane microscopy (MUM) has made it possible to study subcellular dynamics in 3D at high temporal and spatial resolution by simultaneously imaging distinct planes within the specimen. MUM allows high accuracy localization of a point source along the z-axis since it overcomes the depth discrimination problem of conventional single plane microscopy. An important question in MUM experime...
متن کاملAn information-theoretic approach to designing the plane spacing for multifocal plane microscopy.
Multifocal plane microscopy (MUM) is a 3D imaging modality which enables the localization and tracking of single molecules at high spatial and temporal resolution by simultaneously imaging distinct focal planes within the sample. MUM overcomes the depth discrimination problem of conventional microscopy and allows high accuracy localization of a single molecule in 3D along the z-axis. An importa...
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this paper deals with the basic notions of k-tautimmersions . these notions come from two special cases; that is, tight and taut immersions. tight and taut based on high and distance functions respectively and their basic notions are normal bundle, endpoint map, focal point, critical normal. we generalize hight and distance functions to cylindrical function and define basic notions of k-taut im...
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Owing to its sensitivity and noninvasiveness, far-field fluorescence microscopy would be almost ideal for biological imaging if the resolution of its established variants were not limited by diffraction toDr l=ð2n sinaÞ, with l denoting the wavelength of light, n the index of refraction, and a the aperture angle of the objective lens. The first concept to overcome these limits in a fundamental ...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2014
ISSN: 1094-4087
DOI: 10.1364/oe.22.016706